human sarcoma cell lines ht 1080  (ATCC)

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: 72-hour treatment with doxorubicin. Top graphs represent (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell proliferation compared to an untreated control. Significance is denoted by (*) such that P-values are represented by P<0.05 (*), P<0.005 (**), P<0.0005 (***), and P<0.0001 (****). Bottom graphs represent dose-response curve of (E) HT1080, (F) SW872, (G) VAESBJ, and (H) SW982 cell lines with associated IC50, calculated using Prism GraphPad 7.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Efficacy of 72-hour treatment with doxorubicin alone and in combination with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cells measured through proliferation compared to untreated controls. Data are presented as mean ± SD.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell lines. The dotted lines represent thresholds for Chou-Talalay antagonism (CI>1.1) as well as synergy (CI<0.9). Additive effect corresponds to (0.9

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination Index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Mucin depletion as assessed quantitatively through ImageJ RGB analysis

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC1 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC1 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC4 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC4 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Western blot analysis of a panel of biomarkers for apoptosis, cell cycle progression, and autophagy in soft tissue sarcoma HT1080 and SW872 cells.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Western Blot

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: 72-hour treatment with doxorubicin. Top graphs represent (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell proliferation compared to an untreated control. Significance is denoted by (*) such that P-values are represented by P<0.05 (*), P<0.005 (**), P<0.0005 (***), and P<0.0001 (****). Bottom graphs represent dose-response curve of (E) HT1080, (F) SW872, (G) VAESBJ, and (H) SW982 cell lines with associated IC50, calculated using Prism GraphPad 7.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Efficacy of 72-hour treatment with doxorubicin alone and in combination with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cells measured through proliferation compared to untreated controls. Data are presented as mean ± SD.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell lines. The dotted lines represent thresholds for Chou-Talalay antagonism (CI>1.1) as well as synergy (CI<0.9). Additive effect corresponds to (0.9

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination Index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Mucin depletion as assessed quantitatively through ImageJ RGB analysis

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC1 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC1 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC4 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC4 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Western blot analysis of a panel of biomarkers for apoptosis, cell cycle progression, and autophagy in soft tissue sarcoma HT1080 and SW872 cells.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Western Blot